The goal of the research in my laboratory is to further our understanding of the mechanisms involved in gender differences in vascular reactivity under normal conditions and diabetes. We have demonstrated that estrogen modulates contractility of the vasculature through regulation of calcium sensitivity of contractile proteins in smooth muscle. The ability of estrogen to modulate calcium sensitivity is mediated through the Rho/rho-kinase pathway. Estrogen modulates this pathway in diabetics as well to protect vasculature in female animals. We are currently focusing on the mechanisms responsible for estrogen regulation of the Rho/rho-kinase pathway. In addition, we are examining mechanisms responsible for the dysregulation in both Type 1 and Type 2 diabetics. Diabetes is a major risk factor for development of cardiovascular disease. Understanding mechanisms that contribute to vascular dysfunction in diabetes may provide clues for treatment to prevent the progression of cardiovascular disease.
Endothelial CaMKII as a regulator of eNOS activity and NO-mediated vasoreactivity. Murthy S, Koval OM, Ramiro Diaz JM, Kumar S, Nuno D, Scott JA, Allamargot C, Zhu LJ, Broadhurst K, Santhana V, Kutschke WJ, Irani K, Lamping KG, Grumbach IM. PLoS One. 2017 Oct 23;12(10):e0186311. doi: 10.1371/journal.pone.0186311. eCollection 2017.
Role of CaMKII in Ang-II-dependent small artery remodeling. Prasad AM, Ketsawatsomkron P, Nuno DW, Koval OM, Dibbern ME, Venema AN, Sigmund CD, Lamping KG, Grumbach IM. Vascul Pharmacol. 2016 Dec;87:172-179. doi: 10.1016/j.vph.2016.09.007. Epub 2016 Sep 20.
Differential Regulation of Human and Mouse Myometrial Contractile Activity by FSH as a Function of FSH Receptor Density. Stilley JA, Guan R, Santillan DA, Mitchell BF, Lamping KG, Segaloff DL. Biol Reprod. 2016 Aug;95(2):36. doi: 10.1095/biolreprod.116.141648. Epub 2016 Jun 22.
Calcium/calmodulin-dependent kinase II inhibition in smooth muscle reduces angiotensin II-induced hypertension by controlling aortic remodeling and baroreceptor function. Prasad AM, Morgan DA, Nuno DW, Ketsawatsomkron P, Bair TB, Venema AN, Dibbern ME, Kutschke WJ, Weiss RM, Lamping KG, Chapleau MW, Sigmund CD, Rahmouni K, Grumbach IM. J Am Heart Assoc. 2015 Jun 15;4(6):e001949. doi: 10.1161/JAHA.115.001949.
Dietary fat, fatty acid saturation and mitochondrial bioenergetics. Yu L, Fink BD, Herlein JA, Oltman CL, Lamping KG, Sivitz WI. J Bioenerg Biomembr. 2014 Feb;46(1):33-44.
Differential control of calcium homeostasis and vascular reactivity by Ca2+/calmodulin-dependent kinase II. Prasad AM, Nuno DW, Koval OM, Ketsawatsomkron P, Li W, Li H, Shen FY, Joiner ML, Kutschke W, Weiss RM, Sigmund CD, Anderson ME, Lamping KG, Grumbach IM. Hypertension. 2013 Aug;62(2):434-41. doi: 10.1161/HYPERTENSIONAHA.113.01508. Epub 2013 Jun 10.
5HT(2A) and 5HT(2B) receptors contribute to serotonin-induced vascular dysfunction in diabetes. Nelson PM, Harrod JS, Lamping KG. Exp Diabetes Res. 2012;2012:398406. doi: 10.1155/2012/398406. Epub 2012 Dec 30.
RhoA localization with caveolin-1 regulates vascular contractions to serotonin. Nuno DW, England SK, Lamping KG. Am J Physiol Regul Integr Comp Physiol. 2012 Nov 1;303(9):R959-67. doi: 10.1152/ajpregu.00667.2011. Epub 2012 Sep 5.